B662 |
PSB ENRICHMENT BROTH BASE |
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Formula |
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Ingredients |
gms/lit. |
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Peptic digest of animal tissue Sodium chloride Disodium phosphate, anhydrous Monosodium phosphate, monohydrate |
20.00 10.00 18.00 3.00 |
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Final pH (at 25°C) : 7.5 + 0.2 |
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Directions |
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Suspend 51 gms. in 1000ml. distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to room temperature and aseptically add filter sterilized Cycloheximide solution to a final concentration of 100 mg/lit. |
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