B662 

PSB ENRICHMENT BROTH BASE

Formula

Ingredients 

gms/lit.

Peptic digest of animal tissue

Sodium chloride 

Disodium phosphate, anhydrous 

Monosodium phosphate, monohydrate 

20.00

10.00

18.00

3.00

Final pH (at 25°C) : 7.5 + 0.2

Directions 

Suspend 51 gms. in 1000ml. distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to room temperature and aseptically add filter sterilized Cycloheximide solution to a final concentration of 100 mg/lit.

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