B656 

LACTOSE BLUE AGAR

Formula

Ingredients 

gms/lit.

Peptic digest of animal tissue

Casein enzymic hydrolysate 

Sodium chloride 

Lactose 

Bromo thymol blue 

Agar 

3.50

3.50

5.00

15.50

0.04

13.00

Final pH (at 25°C) : 7.0 + 0.2

Directions 

Suspend 40.54 gms. in 1000ml. distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

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