B654

NUTRIENT AGAR pH 6.8 

Formula 

B654 

Ingredients 

gms/lit. 

Peptic digest of animal tissue

Beef extract

Agar

5.00 

 

3.00 

15.00

Final pH (at 25°C) : 

6.8 + 0.2 

Directions

Suspend 23 gms. in 1000ml. distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. If desired, the medium can be enriched with 5-10% v/v sterile defibrinated blood.

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