B638 |
MOTILITY NITRATE MEDIUM, BUFFERED |
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Formula |
B638 |
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Ingredients |
gms/lit. |
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Peptic digest of animal tissue Beef extract Galactose Potassium nitrate Disodium phosphate Agar |
5.00 3.00 5.00 5.00 2.50 3.00 |
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Final pH (at 25°C) |
7.3 + 0.2 |
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Directions |
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Suspend 23.5 gms. in 1000 ml distilled water containing 5ml. glycerol. Heat to boiling to dissolve the medium completely. Dispense in test tubes to make them half full. Sterilize by autoclaving at 15 lbs pressure (121° C) for 15 minutes. Cool quickly in cool running water and allow the tubed medium to solidify in an upright position. |
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