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B636 |
MODIFIED MCBRIDE LISTERIA AGAR BASE |
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Formula |
B636 |
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Ingredients |
gms/lit. |
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Casein enzymic hydrolysate Peptic digest of animal tissue Beef extract Sodium chloride Glycine anhydride Lithium chloride Phenyl ethanol Agar |
5.00 5.00 3.00 5.00 10.00 0.50 2.50 15.00 |
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Final pH (at 25°C) : 7.3 + 0.2 |
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Directions |
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Suspend 46 gms. in 1000ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15lbs pressure (121°C) for 15 minutes. Cool below 50°C. Before gelling, aseptically add sterile defibrinated blood to a final concentration of 5% v/v in B616 and add rehydrated contents of 1 vial of McBride Listeria Supplement to B616/B636. Mix well and pour into sterile petri plates. |
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