B628 

MODIFIED RAPPAPORT VASSILIADIS MEDIUM

Formula

Ingredients 

gms/lit.

Casein enzymic hydrolysate

Sodium chloride

Monopotassium phosphate

Magnesium chloride 6H2O

Malachite green

5.00

8.00

1.60

40.00

0.04

Final pH (at 25°C) : 5.2 + 0.2

Directions 

Suspend 49.2 grams in 1100 ml distilled water. Heat gently if necessary to dissolve the medium completely. Dispense as desired into tubes and sterilize by autoclaving at 10 lbs pressure (115°C) for 15 minutes.

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