B625

MINIMAL BROTH DAVIS WITHOUT DEXTROSE

Formula 

B625

Ingredients 

gms/lit.

Dipotassium phosphate

Monopotassium phosphate

Sodium citrate

Magnesium sulphate

Ammonium sulphate

7.00

2.00

0.50

0.10

1.00

Final pH (at 25°C) : 7.0 + 0.2

Directions 

suspend 10.6 gms. of (B625) in 990 ml. distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Add 10 ml. of sterile 10% Dextrose solution.  Mix well.

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