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B622 |
MILK SALT AGAR BASE |
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Formula |
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Ingredients |
gms/lit. |
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Peptic digest of animal tissue Beef extract Sodium chloride Agar |
5.00 3.00 65.00 15.00 |
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Final pH (at 25°C) : 7.4 + 0.2 |
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Directions |
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Suspend 88 gms. in 900 ml. distilled water. Heat to boiling to dissolve the medium completely. Dispense and sterilize by autoclaving at 15lbs pressure (121°C) for 15 minutes. Cool to 60°C. Aseptically add 10ml of sterile skim milk (10% w/v skim milk powder solution) to every 90ml of basal medium. Mix well and pour into sterile petri plates. |
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