B619 

TRICHOMONAS MODIFIED CPLM MEDIUM BASE

Formula

Ingredients 

gms/lit.

Peptic digest of animal tissue 

Liver digest 

Maltose 

L-cysteine hydrochloride

32.00

20.00

1.60

2.40

Final pH (at 25°C) : 6.0 + 0.2

Directions 

Suspend 56 gms. in 900ml. distilled water. Heat to dissolve the medium completely. Distribute in bottles in 90ml. amounts and sterilize by autoclaving at 10 lbs pressure (115°C) for 10 minutes. Cool to 50°C and aseptically add the following (per 90 ml. of medium)

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