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B586 |
LEPTOSPIRA MEDIUM BASE, KORTHOF MODIFIED |
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Formula |
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Ingredients |
gms/lit. |
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Peptic digest of animal tissue Sodium chloride Sodium bicarbonate Potassium chloride Calcium chloride Monopotassium hydrogen phosphate Disodium hydrogen phosphate |
0.80 1.40 0.02 0.04 0.04 0.24 0.88 |
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Final pH (at 25°C) : 7.2 + 0.2 |
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Directions |
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(1)Preparation of base : Suspend 3.42 gms. in 1000ml. distilled water. Heat to dissolve the medium completely. Distribute in 100ml amounts in flasks. Sterilize by autoclaving at 10 lbs pressure (115°C) for 15 minutes. Cool to 55°C . (2) Preparation of haemoglobin solution : To the rabbit blood clot, after removing the serum, add equal volume of distilled water . Freeze and thaw repeatedly to haemolyse the corpuscles. Sterilize by Seitz or millipore filtration. (3) Complete medium : To 100 ml sterile base ,add sterile 8 ml inactivated blood serum and 0.8 ml sterile haemoglobin solution. Mix thoroughly. Distribute if desired in 2 – 3ml amounts in sterile screw capped Bijou bottles / tubes. Test for sterility by incubation at 37°C |
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