B582 |
LEAD ACETATE AGAR |
|
Formula |
||
Ingredients |
gms/lit. |
|
Peptic digest of animal tissue Proteose peptone Dextrose Lead acetate Sodium thiosulphate Agar |
15.00 5.00 1.00 0.20 0.08 15.00 |
|
Final pH (at 25°C) : 6.6 + 0.2 |
||
Directions |
||
Suspend 36.28 gms. in 1000ml. distilled water. Boil to dissolve the medium completely. Dispense into test tubes sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Allow to cool the tubes in a slanted position to obtain with generous butts. Inoculate pure culture by surface streaking slant and stabbing the butt. |
||