B582 

LEAD ACETATE AGAR

Formula

Ingredients 

gms/lit.

Peptic digest of animal tissue

Proteose peptone 

Dextrose 

Lead acetate 

Sodium thiosulphate 

Agar 

15.00

5.00

1.00

0.20

0.08

15.00

Final pH (at 25°C) : 6.6 + 0.2

Directions 

Suspend 36.28 gms. in 1000ml. distilled water. Boil to dissolve the medium completely. Dispense into test tubes sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Allow to cool the tubes in a slanted position to obtain with generous butts. Inoculate pure culture by surface streaking slant and stabbing the butt.

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