B581 |
LACTOSE LECITHIN AGAR |
|
Formula |
||
Ingredients |
gms/lit. |
|
Casein enzymic hydrolysate Peptic digest of animal tissue Pancreatic digest of heart muscle Yeast extract Corn starch Sodium chloride Lactose Sodium azide Neomycin sulphate L-Cysteine hydrochloride Calcium chloride Egg lecithin Bromo cresol purple Agar |
12.65 5.50 3.30 3.85 1.10 5.50 10.00 0.20 0.15 0.50 0.05 0.66 0.025 15.00 |
|
Final pH (at 25°C) : 6.8 + 0.2 |
||
Directions |
||
Suspend 58.5 gms. in 1000ml. distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. |
||