B580 

LACTOSE GELATIN MEDIUM

Formula

Ingredients 

gms/lit.

Lactose 

Disodium phosphate 

Phenol red 

Gelatin 

10.00

5.00

0.05

120.00

Final pH (at 25°C) : 7.5 + 0.2

Directions 

Suspend 135 gms. in 100ml. distilled water. Heat to dissolve the medium completely and dispense 10 ml amounts in 15x150 mm screw capped tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Just before use, heat to boiling to remove dissolved oxygen and cool rapidly to incubation temperature.

Print This Page

Close Window