B579

MOTILITY NITRATE MEDIUM, BUFFERED

Formula 

B579

Ingredients 

gms/lit.

Peptic digest of animal tissue

Beef extract

Galactose

Potassium nitrate

Disodium phosphate

Agar

5.00

3.00

5.00

1.00

2.50

3.00

 Final pH (at 25°C) 

7.1 + 0.2

Directions 

Suspend 19.5 gms. of (B579) in 1000 ml distilled water containing 5ml. glycerol. Heat to boiling to dissolve the medium completely. Dispense in test tubes to make them half full. Sterilize by autoclaving at 15 lbs pressure (121° C) for 15 minutes. Cool quickly in cool running water and allow the tubed medium to solidify in an upright position.

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