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B576 |
LACTOBACILLUS BULGARICUS AGAR BASE |
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Formula |
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Ingredients |
gms/lit. |
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Casein enzymic hydrolysate Yeast extract Beef extract Dextrose Dipotassium phosphate Tomato juice Polysorbate 80 Agar |
10.00 5.00 10.00 20.00 2.00 2.00 1.00 20.00 |
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Final pH (at 25°C) : 6.8 + 0.2 |
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Directions |
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Suspend 70 gms. in 920ml. distilled water and heat boiling to dissolve the medium completely. Add 80 ml Acetate Buffer (11.355% sodium acetate and 0.99% acetic acid). Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. DO NOT OVERHEAT THE MEDIUM. |
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