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B570 |
KF STREPTOCOCCUS BROTH WITH BCP |
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Formula |
B570 | ||
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Ingredients |
gms/lit. |
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Peptic digest of animal tissue Casein enzymic hydrolysate Yeast extract Sodium chloride Sodium glycerophosphate Maltose Lactose Sodium azide Bromo cresol purple |
5.00 5.00 10.00 5.00 10.00 20.00 1.00 0.40 0.015 |
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Final pH (at 25°C) : 7.2 + 0.2 |
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Directions |
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Suspend 56.4 gms. of B570 in 1000ml. distilled water. Heat to boiling to dissolve the medium completely. Continue heating for 5 more minutes. Dispense in 100ml amounts or as desired in flasks. DO NOT AUTOCLAVE. Overheating will lower the pH and tender the medium less productive. Cool to 50°C and add 1 ml of 1% sterile Triphenyl Tetrazolium Chloride Solution (BF044) per 100 ml sterile medium. Allow to cool to 45°C for making pour plates of B1078. |
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