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B553 |
HOFERS ALKALINE MEDIUM |
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Formula |
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Ingredients |
gms/lit. |
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Mannitol Dipotassium phosphate Magnesium sulphate Sodium chloride Yeast extract Thymol blue Agar |
10.00 0.50 0.20 0.10 1.00 0.0016 15.00 |
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Final pH (at 25°C) : 11.0 + 0.2 |
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Directions |
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Suspend 26.8 gms in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. |
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