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B539 |
GLUCOSE AZIDE BROTH |
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Formula |
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Ingredients |
gms/lit. |
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Peptic digest of animal tissue Yeast extract Sodium chloride Dipotassium phosphate Monopotasium phosphate Dextrose Sodium azide Bromo cresol purple |
10.00 3.00 5.00 5.00 2.00 5.00 0.25 0.03 |
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Final pH (at 25°C) : 6.7 + 0.2 |
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Directions |
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Suspend 30.3 gms in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Dispense 5ml amounts in 16 X 150 mm test tubes and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. For large inocula of 5 ml or more quantities, prepare double strength medium. |
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