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B528 |
FOLIC ACID INOCULUM MEDIUM |
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Formula |
B528 |
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Ingredients |
gms/lit. |
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Peptonized milk Yeast extract Dextrose Monopotassium phosphate Tomato juice (100 ml) Polysorbate 80 |
15.00 5.00 10.00 2.00 5.00 1.00 |
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Final pH (at 25°C) : 6.8 + 0.2 |
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Directions |
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Suspend 38 gms. of B528 in 1000 ml distilled water. Boil to dissolve the medium completely. Distribute in tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool the tubes rapidly in an upright position. |
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