B524

FLUID THIOGLYCOLLATE MEDIUM / with Beef Extract

Formula 

B524

Ingredients 

gms/lit.

Casein enzymic hydrolysate

Yeast extract 

Beef extract

Dextrose 

Sodium chloride 

L-Cystine 

Sodium thioglycollate 

Resazurin 

Agar 

15.00

5.00

5.00

5.50

2.50

0.50

0.50

0.001

0.75

Final pH (at 25°C) :

7.2 + 0.2

Directions 

Suspend 34.75 gms. of B524 in 1000 ml. distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 25 °C and store in a cool dark place preferably below 25° C.

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