B518 

ESCULIN IRON AGAR

Formula

Ingredients 

gms/lit.

Esculin

Ferric ammonium citrate

Agar

1.00

0.50

15.00

Final pH (at 25°C) : 7.1 + 0.2

Directions 

Suspend 16.5 gms. in 1000 ml. distilled water. Heat to boiling with frequent stirring. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45°C and pour into sterile petri plates to depth of 4-5 mm

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