B515 |
ENDO AGAR BASE |
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Formula |
B515 |
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Ingredients |
gms/lit. |
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Peptic digest of animal tissue Lactose Dipotassium phosphate Sodium sulphite Agar |
10.00 10.00 3.50 2.50 12.00 |
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Final pH (at 25°C) |
7.5 + 0.2 |
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Directions |
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Suspend 38 gms. of B515 in 1000ml. distilled water. Add 4 ml of 10% Basic Fuchsin (BF052) in B515. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Mix well before pouring into sterile petri plates. |
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