B515

ENDO AGAR BASE

Formula 

B515 

Ingredients 

gms/lit.

Peptic digest of animal tissue

Lactose 

Dipotassium phosphate 

Sodium sulphite 

Agar 

10.00 

10.00 

3.50 

2.50 

12.00 

Final pH (at 25°C)

7.5 + 0.2

Directions 

Suspend 38 gms. of B515 in 1000ml. distilled water. Add 4 ml of 10% Basic Fuchsin (BF052) in B515. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Mix well before pouring into sterile petri plates.

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