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B509 |
EDWARDS AND BRUNER SEMISOLID MEDIUM |
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Formula |
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Ingredients |
gms/lit. |
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Peptic digest of animal tissue Beef extract Gelatin Sodium chloride Agar |
10.00 3.00 80.00 5.00 4.00 |
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Final pH (at 25°C) : 6.9 + 0.2 |
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Directions |
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Suspend 102 gms. in 1000 ml. distilled water. Heat to boiling to dissolve the medium completely. Dispense in tubes and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool the tubes in an upright position. |
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