B505 |
GLUTAMATE STARCH PHENOL RED AGAR |
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Formula |
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Ingredients |
gms/lit. |
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L-glutamate, sodium Starch soluble Monopotassium phosphate Magnesium sulphate Phenol red Agar |
10.00 20.00 2.00 0.50 0.36 12.00 |
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Final pH (at 25°C) : 7.2 + 0.2 |
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Directions |
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Suspend 45 gms in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C and add 100 IU/ml Penicillin G.sodium salt and if desired 10 mcg/ml Pimaricin. Mix well and pour into sterile petri plates. |
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