B496

UREA BROTH BASE

Formula 

B496 

Ingredients 

gms/lit.

Monopotassium phosphate

Dipotassium phosphate 

Yeast extract 

Phenol red 

9.10

9.50 

0.10 

0.01 

Final pH (at 25°C) : 6.8 + 0.2

Directions

Suspend 18.7 gms. of (B496) in 950 ml. distilled water. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 55°C. Aseptically add 50 ml of sterile 40% Urea solution. Mix well and distribute in 10 ml amounts into sterile tubes. 

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