B496 |
UREA BROTH BASE |
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Formula |
B496 |
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Ingredients |
gms/lit. |
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Monopotassium phosphate Dipotassium phosphate Yeast extract Phenol red |
9.10 9.50 0.10 0.01 |
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Final pH (at 25°C) : 6.8 + 0.2 |
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Directions |
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Suspend 18.7 gms. of (B496) in 950 ml. distilled water. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 55°C. Aseptically add 50 ml of sterile 40% Urea solution. Mix well and distribute in 10 ml amounts into sterile tubes. |
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