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B495 |
P A BROTH |
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Formula |
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Ingredients |
gms/lit. |
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Peptic digest of animal tissue Trytpose Beef extract Lactose Sodium chloride Dipotassium phosphate Monopotassium phosphate Sodium lauryl sulphate Bromo cresol purple |
5.00 9.83 3.00 7.46 2.46 1.35 1.35 0.05 0.0085 |
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Final pH (at 25°C) : 6.8 + 0.2 |
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Directions |
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Suspend 30.5 gms. in 1000ml. distilled water or if desired, suspend 91.5 gms. in 1000 ml. distilled water to prepare a triple strength medium. Dispense 50 ml. volumes into screw capped tubes Sterilize by autoclaving at 15 lbs pressure (121°C) for 12 minutes. |
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