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B490 |
PKU TEST AGAR BASE |
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Formula |
B490 |
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Ingredients |
gms/lit. |
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L-Glutamic acid DL-Alanine Asparagine Dextrose Dipotassium phosphate Monopotassium phosphate Ammonium chloride Ammonium nitrate Sodium sulphate Magnesium sulphate Manganese chloride Ferric chloride Calcium chloride Agar |
0.50 0.50 0.50 10.00 15.00 5.00 2.50 0.50 0.50 0.05 0.005 0.005 0.0025 15.00 |
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Final pH (at 25°C) : 7.0 + 0.2 |
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Directions |
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Suspend 50 gms. in 1000ml distilled water. Heat to boiling to dissolve the medium completely. DO NOT AUTOCLAVE OR OVERHEAT. Cool to 50°C and add Bacillus subtilis spores and 3.3 mg B-2-thienylalanine in (B482). Mix well and pour into sterile petri plates. |
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