B488 

DEOXYCHOLATE CITRATE AGAR, MODIFIED

Formula

Ingredients 

gms/lit.

Peptic digest of animal tissue

Beef extract 

Lactose 

Sodium citrate 

Ferric citrate 

Sodium deoxycholate 

Sodium thiosulphate 

Neutral red 

Agar 

5.00

5.00

10.00

8.50

1.00

5.00

5.40

0.02

12.00

Final pH (at 25°C) : 7.3 + 0.2

Directions 

Suspend 52 gms. in 1000ml. distilled water. Boil to dissolve the medium completely. DO NOT AUTOCLAVE OR OVERHEAT. Excessive heating is detrimental.

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