B485 

DEOXYCHOLATE CITRATE AGAR , AS PER B.P.

Formula

Ingredients 

gms/lit.

Peptic digest of animal tissue 

Beef extract 

Lactose 

Sodium citrate 

Ferric citrate 

Sodium deoxycholate 

Neutral red 

Agar 

10.00

10.00

10.00

20.00

1.00

5.00

0.02

13.50

Final pH (at 25°C) : 7.3 + 0.2

Directions 

Suspend 69.52 gms. in 1000 ml. of distilled water. Boil to dissolve the medium completely. DO NOT AUTOCLAVE. Avoid excessive heating as it is detrimental to the medium.

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