B481 

D NASE TEST AGAR W/ TOLUIDINE BLUE

Formula 

B481

Ingredients 

gms/lit.

Tryptose

Deoxyribonucleic acid (DNA) 

Sodium chloride 

Toluidine blue 

Agar 

20.00

2.00

5.00

0.10

15.00

Final pH (at 25 C) : 7.3 + 0.2

Directions 

Suspend 42.1 gms. of B481 in 1000 ml. distilled water. Heat with frequent agitation to dissolve the medium completely. Sterilize by autoclaving at 12 to 15 lbs pressure (118°C to 121°C) for 15 minutes. Cool to 45°C and pour into sterile petriplates. 

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