B475 

CRYSTAL VIOLET LACTOSE AGAR

Formula

Ingredients 

gms/lit.

Proteose peptone 

Beef extract 

Lactose 

Crystal violet 

Agar 

5.00

3.00

10.00

0.0033

15.00

Final pH (at 25°C) : 6.8 + 0.2

Directions 

Suspend 33 gms. in 1000 ml. distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

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