B463

PARK AND SANDERS ENRICHMENT BROTH BASE

Formula 

B463 

Ingredients 

gms/lit. 

Casein enzymic hydrolysate

Peptic digest of animal tissue

Yeast extract

Dextrose

Sodium chloride

Monohydrogen sodium sulphite

Sodium pyruvate

10.00

10.00

2.00

1.00

5.00

0.10

0.25

Final pH (at 25°C) : 7.0 + 0.2

Directions 

Suspend 28.35 gms. of B463 in 940ml. distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45°C and aseptically add 50ml of sterile defibrinated lysed horse blood and reconstituted contents of 1 vial of park and sanders selective supplement I (BF087) Mix well. Allow it to stand for 4 hours at 31 to 32°C. Add reconstituted contents of 1 vial of park and sanders selective supplement II (BF089) and incubate for 24 hours at 37°C and then at 42°C under a microaerobic atmosphere for additional 40 to 42 hours with agitation at 100 rpm.

Print This Page

Close Window