B461 |
PEIZER TB MEDIUM BASE |
|
Formula |
||
Ingredients |
gms/lit. |
|
Casein acid hydrolysate Beef extarct Sodium glutamate Potato starch Ferric ammonium citrate Magnesium sulphate Dipotassium hydrogen phosphate Citric acid Agar |
5.00 3.00 3.00 15.00 0.10 0.015 3.50 0.10 15.00 |
|
Final pH (at 25°C) : Self |
||
Directions |
||
Suspend 44.72 gms. in 1000ml. distilled water. Mix well and add 40 ml glycerol if desired. Sterilize by autoclaving at 12 lbs pressure (118°C) for 15 minutes. Cool to around 55°C and aseptically add egg yolk emulsion (prepared from 10 sterile egg yolks and 25 ml sterile saline, to which 1 ml of sterile 20% dextrose solution and 13 ml of 1% malachite green solution is added). Mix thoroughly and dispense in tubes, then allow it to solidify as slants. |
||