B461 

PEIZER TB MEDIUM BASE

Formula

Ingredients 

gms/lit.

Casein acid hydrolysate

Beef extarct

Sodium glutamate

Potato starch

Ferric ammonium citrate

Magnesium sulphate

Dipotassium hydrogen phosphate

Citric acid

Agar

5.00

3.00

3.00

15.00

0.10

0.015

3.50

0.10

15.00

Final pH (at 25°C) : Self

Directions 

Suspend 44.72 gms. in 1000ml. distilled water. Mix well and add 40 ml glycerol if desired. Sterilize by autoclaving at 12 lbs pressure (118°C) for 15 minutes. Cool to around 55°C and aseptically add egg yolk emulsion (prepared from 10 sterile egg yolks and 25 ml sterile saline, to which 1 ml of sterile 20% dextrose solution and 13 ml of 1% malachite green solution is added). Mix thoroughly and dispense in tubes, then allow it to solidify as slants.

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