B458 |
PEPTONE SORBITOL BILE BROTH |
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Formula |
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Ingredients |
gms/lit. |
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Peptic digest of animal tissue Sorbitol Disodium phosphate Monosodium phosphate Bile salt mixture Sodium chloride |
5.00 10.00 8.23 1.20 1.50 5.00 |
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Final pH (at 25°C) : 7.6 ± 0.2 |
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Directions |
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Suspend 30.93 gms. in 1000ml. distilled water. Heat to boiling to dissolve the medium completely. Dispense 100 ml into Wheaton bottles. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. |
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