B442 

BUFFERED GLYCEROL SALINE BASE

Formula

Ingredients 

gms/lit.

Sodium chloride 

Dipotassium phosphate 

Monopotassium phosphate 

Phenol red 

4.20

3.10

1.00

0.003

Final pH (at 25°C) : 7.2 + 0.2

Directions 

Suspend 8.3 gms. in 700 ml. distilled water. Add 300 ml of glycerol. Mix well and dispense in screw capped tubes or suitable containers. Sterilize by autoclaving at 15lbs pressure (121°C) for 15 minutes.

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