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B441 |
BROMO CRESOL PURPLE BROTH |
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Formula |
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Ingredients |
gms/lit. |
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Peptic digest of animal tissue Sodium chloride Beef extract Bromo cresol purple |
10.00 5.00 3.00 0.04 |
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Final pH (at 25°C) : 7.0 + 0.2 |
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Directions |
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Suspend 18 gms. in 1000 ml. distilled water. Heat if necessary to dissolve the medium completely. Dispense in tubes containing inverted Durham’s tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 10 minutes. Cool and aseptically add sterile desired carbohydrate to a final concentration of 0.5 – 1.0%. |
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