B430 |
BLOOD AGAR BASE W/ LOW PH ( W/O BLOOD) |
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Formula |
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Ingredients |
gms/lit. |
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Beef heart infusion from – (Beef Extract) Tryptose Sodium chloride Agar |
500.00 10.00 5.00 15.00 |
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Final Ph (at 25°C) : 6.8 + 0.2 |
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Directions |
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Suspend 40 gms. in 1000 ml. distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 50°C and aseptically add 5%v/v sterile defibrinated blood. Mix well and pour into sterile petri plates. |
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