B430 

BLOOD AGAR BASE W/ LOW PH ( W/O BLOOD)

Formula

Ingredients 

gms/lit.

Beef heart infusion from – (Beef Extract) 

Tryptose 

Sodium chloride 

Agar 

500.00

10.00

5.00

15.00

Final Ph (at 25°C) : 6.8 + 0.2

Directions 

Suspend 40 gms. in 1000 ml. distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 50°C and aseptically add 5%v/v sterile defibrinated blood. Mix well and pour into sterile petri plates.

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