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B425 I |
BILE ESCULIN AZIDE AGAR |
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Formula |
B425I |
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Ingredients |
gms/lit. |
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Casein enzymic hydrolysate Peptic digest of animal tissue Yeast extract Oxgall Sodium chloride Esculin Ferric ammonium citrate Sodium azide Agar |
17.00 3.00 5.00 10.00 5.00 1.00 0.50 0.15 15.00 |
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Final pH (at 25°C) : |
7.2 + 0.2 | ||
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Directions |
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Suspend 56.65 gms. in 1000 ml. distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121 °C) for 15 minutes. Caution : Sodium azide has a tendency to form explosive metal azides with plumbing materials. It is advisable to use enough water to flush off the disposables. |
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