B421 |
BILE BROTH BASE |
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Formula |
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Ingredients |
gms/lit. |
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Peptic digest of animal tissue Beef extract Oxgall Esculin Ferric citrate Agar |
5.00 3.00 40.00 1.00 0.50 15.00 |
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Final pH (at 25°C) : 6.6 + 0.2 |
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Directions |
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Suspend 64.5 gms. in 1000 ml. distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121 °C) for 15 minutes. |
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