B421 

BILE BROTH BASE

Formula

Ingredients 

gms/lit.

Peptic digest of animal tissue

Beef extract 

Oxgall 

Esculin 

Ferric citrate 

Agar 

5.00

3.00

40.00

1.00

0.50

15.00

Final pH (at 25°C) : 6.6 + 0.2

Directions 

Suspend 64.5 gms. in 1000 ml. distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121 °C) for 15 minutes. 

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