B420 

PHOTOBACTERIUM BROTH

Formula

Ingredients 

gms/lit.

Casein enzymic hydrolysate

Yeast extract

Sodium chloride

Ammonium chloride

Magnesium sulphate

Ferric chloride

Calcium carbonate

Monopotassium dihydrogen phosphate

Sodium glycerophosphate

5.00

2.50

30.00

0.30

0.30

0.01

1.00

3.00

23.50

Final pH (at 25°C) : 7.0 + 0.2

Directions 

Suspend 65.61 gms. in 1000ml. distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Before pouring mix well to evenly distribute slight precipitate formed.

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