B407

PURPLE BROTH BASE

Formula 

B407 

Ingredients 

gms/lit.

Peptone, special

Sodium chloride 

Bromo cresol purple

10.00 

5.00 

0.02 

Final pH (at 25°C) : 6.8 + 0.2

Directions 

Suspend 15 gms. of B407 in 1000ml. distilled water. Add 5-10 gms. of the carbohydrate to be tested. Dispense in tubes as desired and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Alternatively sterilize the basal medium using 900 ml. distilled water and add 100 ml. separately sterilized 5- 10% solution of the desired carbohydrate to it.

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