B403 

ANAEROBIC AGAR WITHOUT DEXTROSE OR EH INDICATOR 

Formula 

Ingredients

gms/lit.

Casein enzymic hydrolysate

Sodium chloride

Sodium thioglycollate

Sodium formaldehyde sulphoxylate

Agar 

20.00

5.00

2.00

1.00

15.00 

Final pH (at 25°C) : 7.2 + 0.2  

Directions

Suspend 43 gms. in 1000 ml. distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

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