B401 

ANAEROBIC EGG AGAR BASE

Formula

 Ingredients 

gms/lit.

 Proteose peptone

Casein enzymic hydrolysate 

Yeast extract

Sodium chloride 

Agar 

 20.00

5.00

5.00

5.00

20.00

Final pH (at 25°C) : 7.0 ± 0.2

Directions 

Suspend 55 gms. in 1000 ml. distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 50°C and add 80 ml Egg Yolk Emulsion (BF003). Mix thoroughly before pouring into sterile plates.

Print This Page

Close Window