B401 |
ANAEROBIC EGG AGAR BASE |
|
Formula |
||
Ingredients |
gms/lit. |
|
Proteose peptone Casein enzymic hydrolysate Yeast extract Sodium chloride Agar |
20.00 5.00 5.00 5.00 20.00 |
|
Final pH (at 25°C) : 7.0 ± 0.2 |
||
Directions |
||
Suspend 55 gms. in 1000 ml. distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 50°C and add 80 ml Egg Yolk Emulsion (BF003). Mix thoroughly before pouring into sterile plates. |
||