B400 

AMMONIUM PHOSPHATE AGAR 

Formula 

Ingredients 

gms/lit.

Ammonium phosphate

Dextrose 

Potassium chloride

Magnesium sulphate

Bromocresol purple

Agar 

1.00

10.00

0.20

0.20

0.05

15.00 

Final pH (at 25°C) : 7.0 + 0.2 

Directions

Suspend 26.5 gms. in 1000 ml. distilled water. Mix thoroughly and heat to boiling to dissolve the medium completely. Dispense in tubes and sterilize by autoclaving at 12-15 lbs pressure (118-120°C) for 10 minutes. Allow the tubes to cool in slanted position.

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