B400 |
AMMONIUM PHOSPHATE AGAR |
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Formula |
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Ingredients |
gms/lit. |
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Ammonium phosphate Dextrose Potassium chloride Magnesium sulphate Bromocresol purple Agar |
1.00 10.00 0.20 0.20 0.05 15.00 |
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Final pH (at 25°C) : 7.0 + 0.2 |
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Directions |
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Suspend 26.5 gms. in 1000 ml. distilled water. Mix thoroughly and heat to boiling to dissolve the medium completely. Dispense in tubes and sterilize by autoclaving at 12-15 lbs pressure (118-120°C) for 10 minutes. Allow the tubes to cool in slanted position. |
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