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B394 |
ACTINOMYCES BROTH |
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Formula |
B394 |
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Ingredients |
gms/lit |
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Beef heart infusion,solids Tryptose Casein enzymic hydrolysate Yeast extract Dextrose L-cysteine hydrochloride Starch,soluble Sodium chloride Monopotassium phosphate Ammonium sulphate Magnesium sulphate Calcium chloride |
10.00 10.00 4.00 5.00 5.00 1.00 1.00 5.00 15.00 1.00 0.20 0.02 |
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Final pH (at 25°C) |
7.2 + 0.2 |
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Directions |
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Suspend 57.22 gms. of B394 in 1000ml. distilled water .Boil to dissolve the medium completely. Distribute into tubes or flasks. Sterilize by autoclaving at 15 lbs pressure (121 °C) for 15 minutes. |
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