B393

 

ACTINOMYCES AGAR

Formula

B393

Ingredients

gms/lit

Beef heart infusion,solids 

Tryptose 

Casein enzymic hydrolysate

Yeast extract

Dextrose 

L-cysteine hydrochloride

Starch,soluble

Sodium chloride

Monopotassium phosphate

Ammonium sulphate 

Magnesium sulphate 

Calcium chloride 

Aga 

10.00

10.00

4.00

5.00

5.00 

1.00 

1.00 

5.00

15.00 

1.00 

0.20 

0.02

20.00

Final pH (at 25°C)

6.9 + 0.2 

Directions

Suspend 77.22 gms. of B393 in 1000ml. distilled water .Boil to dissolve the medium completely. Distribute into tubes or flasks. Sterilize by autoclaving at 15 lbs pressure (121 °C) for 15 minutes.

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