B387 

LAURYL TRYPTOSE MANNITOL BROTH W/TRYPTOPHAN

Formula

Ingredients 

gms/lit.

Tryptose

Mannitol 

Sodium chloride 

Dipotassium phosphate 

Monopotassium phosphate 

Sodium lauryl sulphate 

L-Tryptophan 

20.00

5.00

5.00

2.75

2.75

0.10

0.20

Final pH (at 25°C) : 6.8 + 0.2

Directions 

Suspend 35.8 gms. in 1000ml. distilled water. Warm gently to dissolve the medium completely. Dispense in fermentation tubes with inverted Durham’s tubes and sterilize by autoclaving at 10 lbs pressure (115°C) for 10 minutes.

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