B362 

YEAST NITROGEN BASE

Formula

Ingredients 

gms/lit.

Ammonium sulphate

L-Histidine hydrochloride 

DL-Methionine 

DL-Tryptophan 

Biotin 

Calcium pantothenate 

Folic acid 

Inositol

Niacin 

p-Amino benzoic acid 

Pyridoxine hydrochloride

Riboflavin (vit B2)

Thiamine hydrochloride 

Boric acid

Copper sulphate 

Potassium iodide 

Ferric chloride

Manganese sulphate 

Sodium molybdate 

Zinc sulphate 

Monopotassium phosphate 

Magnesium sulphate 

Sodium chloride 

Calcium chloride 

5.00

0.01

0.02

0.02

0.00002

0.0004

0.000002

0.002

0.0004

0.0002

0.0004

0.0002

0.0004

0.0005

0.00004

0.0001

 0.0002

0.0004

0.0002

0.0004

1.00

0.50

0.10

0.10

Final pH (at 25°C) : 5.4 + 0.2

Directions 

For best results, the medium should be prepared in 10 X strength. Suspend 6.7 gms. in 100 ml distilled water. Add 5 gms. of dextrose or an equivalent amount of other carbohydrate. Warm if necessary to dissolve the medium completely. Sterilize by filtration. Keep refrigerated until use Final medium is made by pipetting 0.5 ml into 4.5 ml of sterile distilled water.

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