B358

WILKINS CHALGREN ANAEROBIC BROTH

Formula

B358 

Ingredients 

gms/lit. 

Casein enzymic hydrolysate

Peptic digest of animal tissue

Yeast extract

Dextrose

Sodium chloride

L-arginine

Sodium pyruvate

Hemin

Menadione

10.00

10.00

5.00

1.00

5.00

1.00

1.00

0.005

0.0005

Final pH (at 25°C) : 7.1 + 0.2

Directions

Suspend 33 gms. of B358 in 1000ml. distilled water. Heat to boiling to dissolve the medium completely. Dispense and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 50C before adding antibiotics to be tested. Mix gently and pour into sterile petri plates. For cultivation of anaerobes add Non Spore Anaerobic Supplement or G.N. Spore anaerobic Supplement as desired into sterile molten medium before pouring into sterile petri plates.

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