B354

WL NUTRIENT BROTH

Formula 

B354   

Ingredients

gms/lit.

Casein enzymic hydrolysate

Yeast extract

Dextrose

Monopotassium phosphate

Potassium chloride

Calcium chloride

Magnesium sulphate

Ferric chloride

Manganese sulphate

Bromo cresol green

5.00

4.00

50.00

0.55

0.425

0.125

0.125

0.0025

0.0025

0.022

Final pH (at 25°C) : 5.5 + 0.2

Directions

Suspend 60.25 gms. of B354 in 1000ml. distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. If desired, to obtain a pH of 6.5, add 1% solution of sodium bicarbonate

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